Assessment of dispersion metrics for estimating single-cell transcriptional variability
Fig 4
Absolute change in transcriptional variability is not correlated with transcription level, gene length, promoter GC content, and evolutionary gene age.
(A) Absolute change in transcriptional variability at E9.5 and at E11.5 is extremely weakly correlated with transcript levels as measured by average normalized counts. (B) The correlation coefficient of absolute change in transcriptional variability with transcript level varies with the threshold for removing lowly expressed genes at E9.5. Absolute change in transcriptional variability is also extremely weakly correlated with gene length (C), promoter GC content (D), and gene phylostrata (E).