Degradation graphs reveal hidden proteolytic activity in peptidomes
Fig 5
Degradation graph analysis of urinary peptidomes from diabetic and healthy individuals.
a Peptidomic data from urine samples of individuals with diabetes (n = 15) and healthy controls (n = 15) [51] were analyzed without enzymatic digestion using LC–MS/MS. b For each sample, degradation graphs were reconstructed by gradient descent to fit the modeled peptide distribution to observed abundances. c Comparison of total generated versus observed peptide abundance revealed that conventional quantification underestimated proteolytic activity by approximately 3.7-fold in both groups. d Mapping total inflow along the uromodulin sequence showed a localized increase in degradation flow within the biomarker-associated region, highlighting differential proteolysis between healthy and diabetic samples. This figure was made with BioRender.