Putting BASIL in a BLT: A Bayesian filtering method for estimating the fitness effects of nascent adaptive mutations
Fig 4
Properties of the measurement process.
A. Barcode frequency estimated from barcode sequencing data (abscissa) versus the lineage frequency set experimentally (ordinate). Each point is a unique barcode. Error bars show one standard deviation of the mean. The 1-to-1 line is shown in gray. B. Variance of the read count versus the mean read count. Each point represents data from multiple barcodes at the same expected lineage frequency. Shade represents coverage. Solid blue line is the parabola given by equation (2). Other curves are described in the Materials and Methods and their fitted parameter values are provided in S2 Table.