Tutorial for variant interrogation in tumor samples
Fig 2
Flowchart of common sequencing file types and analysis stages.
The diagram illustrates the typical file types encountered throughout the sequencing and analysis pipeline, progressing from raw data (left) to results (right). File types are grouped by processing phase: green (Phase 1: Planning and pre-processing), yellow (Phase 2: Variant calling and annotation), and blue (Phase 3: Filtering and validation). Solid arrows indicate the standard forward progression of file generation, while dotted arrows represent steps where data can be reverted to a previous file type. The objective is to complete the pipeline, transforming raw reads into interpretable variants. Created in BioRender. Arseneau, R. (2026) https://BioRender.com/wx6ql68.