Decoding the role of DNA sequence on protein-DNA co-condensation
Fig 4
DNA sequence heterogeneity leads to the co-existence of multiple protein-DNA condensates at equilibrium.
A Schematic represents heterogeneous DNA II model: two blocks of high-affinity monomers (2.25 kBT, 100 monomers in red) are separated by a block of low-affinity monomers (0.1 kBT, grey) of identical length. Proteins (green) interact with each other with an interaction strength of 2 kBT. B Snapshots shown for cases with two condensates co-existing at equilibrium for all five independent realizations at and
=84.50
. High-affinity monomers, low-affinity monomers, and proteins are shown in red, grey, and green respectively. C Scaled radius of the larger droplet (black) and smaller droplet (red) is shown for all the five trajectories quantifying the arrested coarsening. D Representative kymograph showing visualization of coarsening kinetics for one initial condition.