Cell-DINO: Self-supervised image-based embeddings for cell fluorescent microscopy
Fig 8
Image-based profiling of cellular morphology.
A) Single-cell heterogeneity analysis on the HPA-SC dataset. Only U2OS cells were selected for this analysis to measure morphological differences for three proteins EFHC2, PSTPIP2, and NUSAP, which have been annotated as exhibiting no heterogeneity, spatial heterogeneity, and intensity heterogeneity, respectively. The matrices display similarity of single cells from images labeled for the corresponding proteins using Cell-DINO embeddings. Color images in the bottom illustrate the composite multi-channel visualization, and green images represent the corresponding protein channel. The arrows point to cells that differ in their protein localization activity, explaining the heterogeneity patterns observed. Larger version available in Fig D in S1 Text) Similarity matrix of treatment-level profiles of aggregated Cell-DINO embeddings for 1,571 compounds in the LINCS dataset. Two compound clusters are highlighted with colored mechanism-of-action annotations on the right-hand side. In all matrices, dark blue indicates high similarity, and light blue or white indicates low similarity.