Library size-stabilized metacells construction enhances co-expression network analysis in single-cell data
Fig 3
Microglia gene co-expression modules.
Module names are prefixed with “MC” (abbreviation for microglia) followed by the color label. a) UMAP representation of the co-expression network visualizes individual genes (kMEs > 0.2) with eigengene of each module indicated by larger circles colored by the module color. Positive module eigengene correlations > 0.5 are indicated by grey lines connecting the eigengenes. b) Modules-phenotype association analysis. Correlation between the module eigengene and phenotypes. Numbers indicate correlation coefficients and p-values. Braaksc: Braak Stage is the semiquantitative measure (from no effect on neocortex to severity effect) of severity of neurofibrillary tangle (NFT) pathology. Ceradsc: CERAD score is the semiquantitative measure of neuritic plague (from severity to no effect). Cogdx: Clinical consensus diagnosis of cognitive status at time of death (from no cognitive impairment to dementia). Dcfdx_lv: Clinical diagnosis of cognitive status at last visit (from no cognitive impairment to dementia). c) The biological process gene ontology enrichment analysis of genes. GeneRatio is the ratio between genes of interest in the gene set and total genes of interest. Dot color represents the adjusted p-value (Benjamini–Hochberg method) of enrichment analysis.