Mouse and human striatal projection neurons compared - somatodendritic arbor, spines and in silico analyses
Fig 2
Repairing morphological reconstructions (soma centre as black dot, dendrite centre lines in blue, diameter not up to scale).
(A) Cut points are identified in the (x, y)-plane as dendritic terminal nodes in the vicinity of the maximum value of the z-coordinate of the reconstruction (red dots). Scale bars are 100 μm. (B) Complete dendrites are selected as dendritic branches not containing cut points (shown in blue). (C) The predicted morphology is composed of half of the original morphology artificially cut at the level of the soma and its mirror image flipped along the z-axis (grey lines). (D) Incomplete dendrites are extended using parts of the complete dendrites of the same topological order as the corresponding cut points (shown in red). (E) Validation of the repair process for human SPNs. Distributions of morphometric features of the repaired reconstructions (dark blue, 147 dendrites in 27 reconstructions) is compared to the morphometry of the complete dendrites (light blue, 35 complete dendrites), Z-scores are shown in bold for each morphometric feature.