A compact model of Escherichia coli core and biosynthetic metabolism
Fig 2
The iCH360 model shows similar, but more realistic metabolic capabilities than iML1515.
Considering glucose as a feedstock and studying ethanol, lactate, and succinate production, a production envelope analysis yields similar results in the two models (note that the dashed line representing the production envelope of iML1515 is sometimes hidden behind the coloured lines). Growth rate and production fluxes were computed by limiting the glucose uptake rate to a maximum of 10 mmol/gDW/h, so that the production yield (in moles of product per mole of carbon source) can be obtained by dividing the production flux (y-axis) by 10. In the scenario of acetate production (top right panel) iCH360 avoids an unrealistically high production flux [16] as predicted by iML1515. An extended set of production envelope comparisons between the two models is available online in the code repository supporting this manuscript.