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Using stochastic cellular automata to model and define sufficient conditions for the survival of Enterococcus faecalis biofilms with the pCF10 plasmid under erythromycin treatment

Fig 10

Measurements of protective regions.

(A) Example of measuring the width () of rigid structures in a biofilm slice used for quantifying parameter . Yellow lines register two rigid structures whose lengths are measured and averaged to compute . The microscopy slice is of a 3-day, 1-hour treated biofilm using method outlined in Sect 4.2. (B) Red boxes encompass protective structures while the area between the two boxes would be the protected region. Under treatment, protective structures buffer and conserve the protected region allowing continued less densely packed, unstressed growth. (C) Example of measuring the distance between four cells that are not chained or part a rigid structure to estimate L.

doi: https://doi.org/10.1371/journal.pcbi.1013425.g010