Using stochastic cellular automata to model and define sufficient conditions for the survival of Enterococcus faecalis biofilms with the pCF10 plasmid under erythromycin treatment
Fig 2
Image processing steps and results.
(A) Microscopy image stack. (B) Post connection algorithm and removal of rigid base. (C) Microscopy x-slice example containing a protected region. (D) Corresponding x-slice in workflow. (E) Density-based clustering of x-slice with marked centers of mass. (F) Average volume of protective regions per replicate. Protective structures on average double during treatment. Double star significance indicates a p-value <0.01. (G) Percentage of x-slices containing a protected region per replicate, which remains unchanged during treatment. Image processing data results from 3 biological repeats with 2-3 replicates per experiment.