An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases
Fig 8
Computational Simulations of tRNA Charging and Translation in vivo.
(A) The kinetics of tRNAcys charging by cysRS during translation. (B) The kinetics of tRNAhis charging by hisRS during translation. (C) The movement of tRNA during the translation cycle. Uncharged tRNAs (black) are charged by AARS enzymes (blue) and released as aa-tRNA (red). The aa-tRNA then binds to EfTu:GTP to form ternary complex (green) which is recruited to the ribosome. Ribosomes (purple) incorporate the amino acid into the peptide chain releasing EfTu:GDP and uncharged tRNAs. (D) Partitioning of the total amount of tRNA in the cell. Percentage of total tRNA as free uncharged tRNAs (black), free charged tRNAs (red), free tRNAs in ternary complex (green), and tRNA bound to either ribosomes (purple) or AARS enzymes (blue) is shown as a pie chart at a growth rate of (
min).