An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases
Fig 4
Kinetic model of class II hisRS tRNA charging.
(A) Range of kcat and Km values determined by in vitro pyrophosphate and aminoacylation assays (black dots) are compared with the values of the kinetic model (green triangles). (B) Pre-steady state kinetics simulation with starting concentrations of M, [ATP] = 5 mM and
M and
M show no burst of his-tRNA charging as observed experimentally in [17]. (C) Single turnover simulation of the transfer rate (ktran) and (D) of the overall chemistry step (kchem) show excellent agreement with experimentally observed values in Ref. [17].