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An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases

Fig 3

Kinetic model of class I cysRS tRNA charging.

(A) Range of k_cat and K_m values determined by in vitro pyrophosphate and aminoacylation assays (black dots) are compared with the values of the kinetic model (green triangles). (B) Pre-steady state kinetics simulation with starting concentrations of M, [ATP] = 5 mM and M and M show an initial burst of cys-tRNA charging followed by a slower steady state recapitulating what was observed experimentally in Ref. [9]. (C) Single turnover simulation of the transfer rate (k_tran) and (D) of the overall chemistry step (k_chem) show excellent agreement with experimentally observed values in Ref. [9].

doi: https://doi.org/10.1371/journal.pcbi.1013353.g003