An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases
Fig 2
Computer simulation of pyrophosphate exchange and single turnover kinetics.
(A) Reaction scheme used to simulate pyrophosphate exchange kinetics and, (B) reaction scheme used to simulate single turnover kinetics involving the transfer step. State notation follows that of Fig 1. (C) Example Woolf-Hanes plot and the resulting values of and kcat from a computer simulation of pyrophosphate exchange for E. coli cysRS. (D) Example plot from a computer simulation of single turnover kinetics for E. coli cysRS. Black squares represent data points from the computer simulation while the red curve gives the best fit exponential
to the data.