PoweREST: Statistical power estimation for spatial transcriptomics experiments to detect differentially expressed genes between two conditions
Fig 5
Local power estimation based on the CRC dataset by XGBoost.
The power is fitted by XGBoost under constraints so that it monotonically increases with the percentage of expressed spots, the logFC and the number of slice replicates. The figures are based on power values where the logFC is between 0.1 and 1 and percentage of expressed spots is between 0.05 and 0.15. (A) The fitted power surfaces for DE analysis within the carcinoma border with the number of slice replicates per group being 2, 4, and 6. (B) The relationship between the power and logFC with slice replicates ranging from 1 to 10 for the percentage of spots with the detected gene being 0.05, 0.10, and 0.15.