Evolutionary divergence of induced versus constitutive antiviral gene expression levels between primates and rodents
Fig 8
Divergence in dsRNA response across different primate and rodent species.
(A) Top: logFC distribution of differential expression analysis in dsRNA-stimulation condition between human and mouse (left) and rhesus macaque and rat (right) from the 4-species system. Separation in both boxplots is according to the groups originally defined from human-mouse divergence: “mouse constitutive-low”, “human constitutive-low”, “All other DE genes” (as defined in Figs 3 and 4). The right panel, which is based on rhesus versus rat data shows that the orthologs of two species diverge in a similar manner to the human and mouse orthologs. Thus, both left and right panels use the same three groups of orthologous genes, but the FC values are based on either human-mouse (left) or macaque-rat (right) DE analysis. Bottom: The same as in Top, but with DE preformed between human and mouse in basal conditions and showing “human constitutive-high”,”mouse constitutive-high” and “all other DE genes”. (B) The same as in A, but comparing the human-mouse divergence in response to dsRNA from the 4-species system to each one of the 9 primates-mouse divergence in response to dsRNA from the 10-species system (the primate species used is shown in the bottom, and its data is always compared to mouse). In both (A) and (B) we observed that the patterns of relative FC between the compared groups recapitulate the patterns from the human-mouse dsRNA comparison. In all analyses, FDR-corrected P-values are shown for one-sided Mann–Whitney test, performed under the hypothesis that the FC distribution of the left group is higher than the right group in each of the analyses.