Evolutionary divergence of induced versus constitutive antiviral gene expression levels between primates and rodents
Fig 5
Hierarchically clustered heatmaps based on gene expression levels.
Hierarchical clustering of gene expression data from all human and mouse individuals in the 4-species system in both stimulated and unstimulated conditions. In each panel, one the four previously defined divergent genes groups is shown, with expression levels in the human and mouse orthologs: (A) ‘human constitutive-low’, (B) ‘mouse constitutive-low’, (C) ‘human constitutive-high’ and (D) ‘mouse constitutive-high’. Columns are colored by species (blue and orange for human and mouse, respectively) and by conditions (dark and light for stimulated and unstimulated conditions, respectively) and represent one individual each. Gene levels are shown on a scale based on log(TPM). The clustering indicates that genes have similar levels in three ‘types’ of data, while the fourth is significantly different. For example, in A, both stimulated and unstimulated human samples cluster with the mouse unstimulated samples, while the stimulated mouse is the outgroup, with significantly higher levels of this gene set. This agrees with the patterns observed in Fig 4. The silhouettes were created in BioRender.(2025) https://BioRender.com/i59u303.