Evolutionary divergence of induced versus constitutive antiviral gene expression levels between primates and rodents
Fig 2
A cross-species in vitro immune stimulation analysis shows an overall similarity of transcriptional response across primate and rodent cells with accumaltive diveregnce.
(A) Phylogenies of the two cross-species fibroblast dsRNA-stimulation systems used in this analysis. Top: 4-species stimulation system: Dermal fibroblasts from two primates and two rodents were profiled with and without dsRNA stimulation using RNA-seq, along with ChIP-seq of histone marks and IFNB stimulation profiling [11]. Bottom: 10-species stimulation system: Dermal fibroblasts from nine primates and mouse were profiled with and without dsRNA stimulation using RNA-seq [19]. (B) Scatter plot of principal component analysis (PCA) of expression levels (count values from Salmon mapping [55]). The proportion of variance explained by the principal components is indicated in parentheses. (C) Hierarchical clustering of pairwise Spearman’s rank correlations between fold change in response to dsRNA-stimulation values in 1-to-1 orthologous genes that are DE in at least one of the species (2,771 and 7,711 genes, top and bottom, respectively). The silhouettes were created in BioRender.(2025) https://BioRender.com/i59u303.