Mechanistic modeling of cell viability assays with in silico lineage tracing
Fig 5
Rapidly Dividing Phenotype in Control and Trametinib-Treated Conditions.
A. Histogram displaying low (0-6) moderate (6-16) and high (16+) number of division events for simulated cells under control conditions. Cells (4,000) were compiled across 4 drugs (0 dose), 100 cells per replicate, 10 replicates. B. Histogram displaying low (0-6) moderate (6-16) and high (16+) number of division events for simulated cells under trametinib treatment conditions (0.03 nM). Cells (1,000) were compiled across one dose, 100 cells per replicate, 10 replicates. C. Setup of the hypothesis, relating initial conditions of Generation 1 mother cells to the eventual number of divisions arising from them. D. Principal component plot of the initial states matrix under control conditions. Points are sized by number of division events, with colors equivalent to panel A. E. Top 20 loadings of the second principal component under control conditions, and a cartoon schematic of where they fall along the pathway driving the cell cycle. F. Projection of the trametinib data set onto the principal components learned from the control dataset. Points are sized by number of division events, with colors equivalent to panel A.