GABAergic neurons can facilitate the propagation of cortical spreading depolarization: experiments in mouse neocortical slices and a novel neural field computational model
Fig 2
Effects of the modulation of GABAergic neurons activity and synaptic transmission on CSD propagation speed.
(A) Schematic representation of the effects of optogenetic illumination (BLUE LIGHT) on GABAergic neurons activity and synaptic transmission, and of the effects of isoguvacine (ISO) and gabazine (GBZ) on GABAergic synaptic transmission. Panel A was created in BioRender. Mantegazza, M. (2025) https://BioRender.com/90to28g. (B) CSD propagation speed in WT and VGAT-ChR2-tdtomato expressing slices (pooled) in control (CTRL, same data as in Fig 1); in WT slices perfused with isoguvacine (ISO) or gabazine (GBZ); and in VGAT-ChR2-tdtomato expressing slices exposed to blue light in control (LIGHT) or with gabazine (GBZ + LIGHT). The number of slices (n) is indicated below the x axis. One-way ANOVA (p<0.0001) with Tukey’s post hoc test; the bars indicate significant differences, all with p<0.0001.