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Uncertainty-aware traction force microscopy

Fig 9

TFM-UQ applied to endothelial monolayer experiment demonstrates uncertainty propagation.

(A) Membrane labeling of HUVEC monolayers with CellMask. (B) Corresponding fluorescent bead image (beads of size ). Arrows indicate bead-related image artifacts (C) PIV-UQ displacement field (D) PIV-UQ uncertainty map, . White regions indicate “bad” PIV windows that were deleted and replaced as described in § 2.2. Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of bootstrapped PIV-UQ distribution. (E) Inferred mean marginal posterior traction stress, (F) Marginal posterior traction stress uncertainty field (). Uncertainty arrows denote the pointwise angular uncertainty corresponding to 1 circular std. dev. of marginal posterior . Scale bar :

Fig 9

doi: https://doi.org/10.1371/journal.pcbi.1013079.g009