Statistical signature of subtle behavioral changes in large-scale assays
Fig 8
Two genetic lines of interest, each subjected to two different stimulus intensities: high intensity as previously illustrated, and low intensity, involving a less powerful air puff.
We provide characterizations of each line and protocol. The columns correspond to (1) the control line, (2) R68B05 and (3) R20F11. Row A displays light microscopy images of larval brains expressing the selected GAL4 lines. In Row B, the proportions of each stereotypical action during the 2 seconds following the stimulus, with high intensity in plain color and low intensity in dashed lines. Row C, witness function between latent distributions, highlighting the main sources of behavioral differences between the two protocols for the control and the two lines. Row D, the position of high intensity in red and low intensity in blue in the 2D representation of the geometric relationships between lines, encoded by the distance matrix (as shown in Fig 6B, C). Row E, the positions of high intensity (red) and low intensity (blue) displayed in the hierarchical clustering tree (presented here in circular form)