Statistical signature of subtle behavioral changes in large-scale assays
Fig 7
Samples of genetic lines of interest, “Hits”, with their characterization.
These lines lead to subtle modifications in behavior and were not detected by previous approaches. We present four new hits: two hits associated with complex alterations of the learned latent space and two lines associated with significant sequence deviations from the generative model and the reference. The columns correspond to 1. control line attP2, 2. R68B06, 3. R57F07, 4. R18A10, 5. R38H09. Row A: Light microscopy images of larval brains expressing the selected GAL4 line. Note that there is no picture for attP2, as it is the reference and thus labels no neurons. Row B: Proportions of each stereotypical action evoked during the 2 seconds after stimulis. Row C: Latent distribution of behaviors of the lines, during the 2 seconds after stimulus, with the distribution of the reference line shown in red and the distribution of the hit lines shown in blue. Row D: Witness function between latent distributions of the hit and reference lines, highlighting the main sources of behavioral differences. Note the complex patterns in the latent space, showing these hits do not stem from simple variations in one action. Row E: Z-scores of sequences of three actions between the generative model and experimental sequences. Row F: Position of the reference and hit lines in the 2D representation of the geometric relationships between lines encoded by the distance matrix (shown in Fig 6D,E). Row G: Position of the line in the hierarchical clustering tree (shown here in circular form).