Statistical signature of subtle behavioral changes in large-scale assays
Fig 6
A. Illustrative example of a suffix tree obtained from three larvae performing three different sequences.
Larva 1: ABA, Larva 2: BAC, Larva 3: BD, the seven paths from the root to the leaves correspond to the seven suffixes: A, BA, ABA, AC, BAC, D and BD. Each node shared by at least two larvae is shown in circles: A, B and BA. B. Hierarchical clustering based on the cosine similarity between the suffix tree vectors of each genetic line. Each color is associated with a different cluster. C. Distance matrix representing the squared MMD between all lines from the inactivation screen, computed in a 10D learned latent space for a 2-second time window. D. 2D representation of the geometric relationships between lines, obtained using supervised UMAP [63], encoded by the distance matrix. The bar plot associated with each cluster represents the average variation of behavior during the 2-second window in the six actions behavior dictionary. The thickness of the lines linking two clusters is associated with the coupling between the clusters. E. The z-score distributions’ standard deviation normalises average z-scores between data and generated sequences. We display only the 30 highest values. F. The 17 sequences of nodes with the highest frequency of occurrence for each of the eight clusters.