lncreased risk of slippage upon disengagement of the mitotic checkpoint
Fig 2
Adaptation dynamics with constant unattached kinetochores.
A) Definition of adaptation in the experimental system. UK stands for unattached kinetochores. B) Cumulative distribution of mitotic arrest length. Cells growing in YPD are arrested in G1 and released in nocodazole, indefinitely. Cells carry Mad2-GFP to record checkpoint activation, and Clb2-mCherry to record both mitotic entry (Clb2 rise time) and mitotic exit (Clb2 degradation time). Length of mitotic arrest is defined as the difference between mitotic exit and mitotic entry. C) Definition of adaptation in the model. Adaptation occurs when the system transits from the ON to the OFF state with unattached kinetochores. D) Stochastic simulations of APC/CCdc20 dynamics with fixed number of unattached kinetochores (nuk=10). Reactions in Table 3, parameters in Table 2, initial conditions in Table 1 (checkpoint ON). Filled circles indicate the time APC/CCdc20 crosses the activation threshold (80 mol/cell), after which we assume entry into anaphase. E) Cumulative distribution of simulated mitotic arrests, computed as the difference between the start of the simulation and the time APC/CCdc20 crosses the activation threshold. Representative trajectories are shown in panel (D).