Reliability of plastid and mitochondrial localisation prediction declines rapidly with the evolutionary distance to the training set increasing
Fig 4
Cross-organelle errors in proteome prediction due to physio-chemical properties of the NTS.
Cross organelle prediction errors could be either because an in-vivo plastid protein is in-silico mitochondria localised (a) or vice versa (b). The overlaps between cross-organelle in-vivo and in-silico proteomes identifies these predictions errors. Analysis of the first 20 amino acids of pNTSs incorrectly predicted to be mitochondrial (c) and vice versa (d). Average charge and phosphorylatable amino acids for NTS from all verified organelle proteins of each species are indicated by vertical green (pNTS) and orange (mNTS) lines. Error bars indicate standard error of mean (N = 4–331, S2 Fig). (e) Overlap between predicted (left) and experimentally localised (right, in grey) dual targeted proteins. (f) Predicted (in-silico) intracellular localisation of experimentally verified (in-vivo) dual targeted proteins (left column) and experimentally verified (in-vivo) intracellular localisation of proteins that are predicted (in-silico) to be dual targeted (right column).