Reliability of plastid and mitochondrial localisation prediction declines rapidly with the evolutionary distance to the training set increasing
Fig 2
Performance of algorithms outside the training species.
Comparison of predicted versus experimentally localised plastid (a) and mitochondrial (b) proteome numbers. Each Venn diagram of the top panel shows an overlap between predicted (left circles, colour-coded based on the algorithms used) and experimentally verified organelle proteomes (right circles, grey). The underscored numbers in the bottom corners show the total number of predicted (bottom left) and experimentally confirmed proteins (bottom right). The numbers of proteins that overlap (true positives) are provided in the top right corner in bold, while the numbers of non-overlapping false positives and negatives are shown next to each circle. See also the key for the Venn diagrams on the bottom left. Sensitivity, specificity and precision of individual algorithms and their combinations for plastid (c) and mitochondria (d).