Simulation of 69 microbial communities indicates sequencing depth and false positives are major drivers of bias in prokaryotic metagenome-assembled genome recovery
Fig 4
Student’s Bonferroni adjusted t-test comparing True Positives Species recovered and in the original communities between all pipelines used (8K, DT and MM) according to Taxonomic distribution (Not related; Closely related; Very closely related) and species abundance distribution (Logarithmic decay; Exponential decay; Logarithmic decay with abundance plateaus; Exponential decay with abundance plateaus).
The sequencing depth of the communities was kept at 60 million reads. (*P-value < 0.05).