BoReMi: Bokeh-based jupyter-interface for registering spatio-molecular data to related microscopy images
Fig 3
a) Left: Non-linear manipulations control panel and functionalities. Manipulations are performed through mouse-interactions after activating the “point-select” widget; Right: Example for adjusting the position of slightly disconnected data points (possibly due to a tear) using BoReMi’s elastic non-linear transformation, building on the linear registration in Fig 2. The dragged cells as well as the “threshold of influence” (here set to 500 μm) are indicated by dashed circles. Arrows indicate the drag direction. b) Example for highly precise alignment which allows cell matching between cell-segmented data (as in panel a) and images (here DAPI) of the same tissue section by high magnification (zoom-in) and fine positional adjustment.