BoReMi: Bokeh-based jupyter-interface for registering spatio-molecular data to related microscopy images
Fig 2
Exemplary registration of MERFISH data to database HE images using BoReMi’s linear transformations.
a) BoReMi’s data/image view panel showcasing the different data/image manipulations enabling the registration of MERFISH data and HE images of mouse brain coronal sections. Panel i shows the unmanipulated data as they are obtained from their respective sources. Panels ii-ix show the data after consecutive application of the respective manipulations. b) BoReMi’s control panel that allows parameter selection for the data/image manipulations as shown in panel a):ii: Data point display size, iii: Data subsetting by cluster selection, iv: Scaling by setting the original resolution for data and image as well as the desired common display scale,v: Data orientation change by flipping, vi: Rotation by setting the angle, vii: Pan and zoom, viii: Translocation by setting x and y changes, and ix: Microscopy image selection. vii (pan and zoom) is controlled through mouse-interaction with the view panel. x shows the undo-redo buttons. xi shows the download buttons for the registered data and image. xii shows the data coordinate live-preview.