DNA spontaneously wrapping around a histone core prefers negative supercoiling: A Brownian dynamics study
Fig 5
Multiple nucleosomes assembling.
(A)Distribution of nucleosome assembling distance along a DNA chain at length of 1000 bp. The first nucleosome is constrained at z = 20nm, and then the second histone is wound by DNA to form a new nucleosome. The second nucleosome tends to successfully assemble near the first nucleosome. The distribution of the distance d between two nucleosomes was obtained from 100 simulation trajectories of 100 μs each. (B)Average number of nucleosomes. An initially naked DNA chain with different linking numbers interact with 6 histone cores and form multiple nucleosomes. Compared with the DNA without supercoil (ΔLk = 0), the highly supercoiled DNA (ΔLk = - 5) enhances efficiency of the nucleosome assembling. Each line is the average over 10 simulation trajectories with 75 μs of each.