Alternate conformational trajectories in ribosome translocation
Fig 5
Alternate translocation trajectories of tRNA motion.
(a) Deacyl tRNA motion during forward swivel was tracked by its distance to the E loop (h23, G691-A695). (b) Relative free energy (ΔG*) landscapes of the forward swivel (PRE to INT3) stage of translocation with head domain swivel and E loop-Deacyl tRNA distance as reaction coordinates. Heat maps are obtained by normalizing the number of frames in the targeted MD simulation, where the frame count is divided by the maximum frame count. Shown are the maps for the wild type and seven representative hinge variants active in vitro and in vivo. Maps correspond to ten trajectories per genotype.