Estimating error rates for single molecule protein sequencing experiments
Fig 2
Demonstration of the relationship between fluorosequencing data and the theoretical model.
(A) Illustration of a potential path through the states in the model, in this case the path represents perfect sequencing with no errors. (B) Illustration of the same peptide being sequenced with successive Edman cycles, aligned with the images in (C) the raw data we wish to analyze, i.e. the light emitted from a single molecule measured across two fluorescent channels (rows) for 5 Edman cycles (columns).