Computational design of Periplasmic binding protein biosensors guided by molecular dynamics
Fig 4
Comparison of fluorescence of designed sensors at varied maltose concentrations.
On the left of the axes are the control conditions. cpGFP: the fluorescent protein alone. MBP: the Maltose Binding Protein alone. 131 and 195: cpGFP inserted at MBP’s positions 131 and 195, which are both in areas of flat Pearson space so are predicted to generate poor quality sensors. 170 and 348: cpGFP inserted at MBP’s positions 170 and 348, both very strong sensors previously identified by Nadler et al. [12]. Presented are the means and standard deviations of 3 biological repeats per condition. Tables for all values are in supplement. A) Raw fluorescence of sensors at 0, 1, and 100 mM maltose. B) Relative change in fluorescence from 0 mM maltose to 1 or 100 mM, calculated by (F0—FX) / F0. 0.25 and -20.5 are indicated with dashed lines.