Reliable ligand discrimination in stochastic multistep kinetic proofreading: First passage time vs. product counting strategies
Fig 3
The simplified model of KPR in DNA replication with only one enzyme.
Here, the enzyme (e.g., DNA polymerase) has three states, namely, free (E), bound to correct substrate (ES), and bound to incorrect substrate (ES′). The transition rates between these states are specified in the model. When the enzyme is bound to substrate, it produces the product (P or P′) after a waiting time τ.