Mechanistic computational modeling of monospecific and bispecific antibodies targeting interleukin-6/8 receptors
Fig 5
Simulated Binary (Ab-R), Ternary (R-Ab-R), and Total Bound (Binary + Ternary) levels of bispecific BS1-Receptor complexes, over a range of antibody doses and receptor expression levels.
In these simulations, IL-6R and IL-8R are present in a 1:1 ratio, and simulations were performed for 24 hours after antibody dosing. A-B, Fraction of total BS1 concentration in free (unbound) state for different levels of initial BS1 (A) and receptor (B). C-D, Fraction of total receptor (IL-6R + IL-8R) in different forms/complexes for different levels of initial BS1 (C) and receptor (D). E, Bound receptor fraction across different levels of initial BS1 and receptors. The color indicates the fraction of the total receptor (IL-6R + IL-8R) that is bound to BS1 in each antibody-receptor complex type. A similar heat map for different tocilizumab and 10H2 concentrations is included in the Supporting Information (S9 Fig).