Mechanistic computational modeling of monospecific and bispecific antibodies targeting interleukin-6/8 receptors
Fig 3
Model simulation results using the best-fit parameter set compared to the experimental data used to fit the model parameters.
Simulations were performed under the same conditions as the experiment: 105 cells/well, receptor expression levels from the transduced cell lines (Table 1), and with a 2-hour initial association period followed by a 15-minute free antibody washout. The model simulation results (lines) are compared to the equivalent experimental data (dots). Simulations beyond the range of antibody concentrations used in the experimental data are indicated with dashed lines. Experimental data was not obtained for the combination of tocilizumab and 10H2, but simulations are presented here for comparison. Model output and experimental data are each normalized to the bound BS1 concentration from the initial antibody concentrations where binding reached saturation. The error bars depict the standard error from three experimental replicates; the experimental data was previously published [28]. Simulation results with all obtained parameter sets are included in the Supporting Information (S7 Fig).