Cholinergic modulation supports dynamic switching of resting state networks through selective DMN suppression
Fig 2
Experimental and modeling methods.
A. Experimental framework. Chemogenetic tools (DREADDs) were used to selectively increase cholinergic activity in rat’s basal forebrain (BF). Resting-state fMRI scans were performed during the resting state/after injection of saline and after the injection of CNO, resulting in upregulated cholinergic release in BF. Functional connectivity and other signal features were collected to compare both conditions. B. Simulation framework. The structural connectome of DMLN is the backbone of simulation dynamics. The total synaptic input of all neurons within each area is measured and used for conversion to a BOLD signal. The correlation of BOLD between areas was used to define functional connectivity of DMLN in two conditions—resting saline baseline and increased cholinergic release.