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Versatile multiple object tracking in sparse 2D/3D videos via deformable image registration

Fig 5

Results for tracking posture of a behaving mouse in 2D.

We compare performances of ZephIR and DeepLabCut on tracking 10 body parts that characterize the mouse’s posture over time. A: Accuracy (average percentage of keypoints correctly tracked in unannotated frames) and precision (average distance between predicted position and ground truth position of keypoints) vs the number of manually labeled or ground truth frames. These labeled frames are used as reference frames for ZephIR and as training data for DeepLabCut. The frames are selected based on automated recommendations from each algorithm, meaning the two sets of frames used may not be identical. The last data point (results with 200 training frames) for DeepLabCut are produced with training data generated by verifying and correcting ZephIR results with 10 reference frames. Note that ZephIR achieves better accuracy when only a few labeled frames are provided, but DeepLabCut ultimately reaches a higher accuracy when its training data was augmented with ZephIR. B, C: DeepLabCut and ZephIR results with 20 labeled frames (vertical line in panel A) for tracking mouse body parts as it raises its paws. Note that ZephIR is more stable during motion while DeepLabCut tends to jump between the different body parts. Table: Annotation and computation speed comparison. Annotation time is calculated for the same person, using the respective GUI’s provided with each software package. Training and inference times are tested on the same CPU and single GPU environment and with 20 reference frames (vertical line in panel A). While DeepLabCut is faster for inference, it requires a slow training phase, dramatically increasing the total computation time. This data was produced and provided by the Churchland Lab (UCLA). Raw data is available at: https://ibl.flatironinstitute.org/public/churchlandlab/Subjects/CSHL047/2020-01-20/001/raw_video_data/.

Fig 5

doi: https://doi.org/10.1371/journal.pcbi.1012075.g005