Removing direct photocurrent artifacts in optogenetic connectivity mapping data via constrained matrix factorization
Fig 6
Validating photocurrent subtraction via simulated-plus-real “hybrid” datasets.
a, Using a sparsely expressing preparation, we mapped synaptic connectivity using the grid design. We then added simulated photocurrents to these recorded PSCs in order to validate the low-rank model. b Ground truth PSC map, observed map containing real PSCs and simulated photocurrents, and map after subtraction. Bottom right shows the true vs. estimated responses, before and after applying PhoRC. c, Example traces corresponding to the maps shown in c. Subtracted PSCs closely mirror ground-truth. d,e Same as b,c for a second hybrid dataset.