Decomposing bulk signals to reveal hidden information in processive enzyme reactions: A case study in mRNA translation
Fig 7
Fitted IFIs obtained from the analysis of the fluorescence signatures of truncated LepB mRNAs of different lengths.
The IFIs correspond to the different states of the translation process (see Fig 8 and [8]). The lowest IFI (IFI5) is associated to the states after binding of Phe and before binding of the second Ala to the nascent peptide chain. The IFIs associated to the artificial state of a ribosome at the end of a truncated mRNA are not shown.