Decomposing bulk signals to reveal hidden information in processive enzyme reactions: A case study in mRNA translation
Fig 3
Fitting fluorescence signatures of longer mRNAs reveals signs of an ill-posed problem.
a): Fluorescence signature of an mRNA that consists of 26 identical codons for a random IFI input vector. The simulated fluorescence signature is compared to the best theoretical fit in terms of least squares. No regularization is applied. b): Fitted IFIs obtained from the decomposition of the fluorescence signature in a) compared to the given IFI input vectors. No regularization is applied.