A genome-wide comprehensive analysis of nucleosome positioning in yeast
Fig 7
Chromatin remodelers maintain nucleosome organisation on a local and far-reaching scale.
Top: RSC (green ellipse) establishes independent nucleosome phasing on each gene (two vertical dashed lines) by maintaining the NDR through positioning the +1 (cornered arrow) and -1 nucleosome. The ATP-dependent positioning is symbolised by black arrows pointing away from RSC. The local remodeling effect of Chd1 (blue ellipse) allows chromatin arrangement independent of Pol II transcription (yellow ellipse). Bottom: in rsc8 strains, the NDR cannot be maintained anymore, and phasing in and outside a gene interfere with each other (single dashed line). We propose that this should equally lead to an increased interdependence of other nuclear processes such as transcription. If chd1 is deleted, nucleosome arrangement is more sensitive to the presence of other large complexes, such as Pol II. During transcription, Pol II is affecting the local positioning (black arrows from Pol II).