Novel metrics reveal new structure and unappreciated heterogeneity in Caenorhabditis elegans development
Fig 5
Branch Distance reveals structure in the AB lineage.
A) Heatmap showing the intersection branch distance between every pair of sublineages in every pair of 21 wild type embryos. B) Illustration of the first two Notch signaling events in the early AB lineage. C) Heatmap showing a zoomed in view of the intersection branch distance between the 21 wild type embryos for each pair of AB-derived sublineages. Colormap is scaled from 0 to the max intersection branch distances between same-generation AB sublineages. D) Heatmap showing a zoomed in view of the intersection branch distance between AB-derived sublineages of 6 embryos treated with RNAi against glp-1 for each pair of AB-derived sublineages. Colormap is scaled from 0 to the max intersection branch distances between same-generation AB sublineages. E) Distributions of intersection branch distances between subsets of AB-derived sublineages in WT embryos and embryos treated with RNAi against glp-1. P-values calculated using 106 iterations of a permutation test.