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OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images

Fig 5

Object-based analysis reveals hidden relationships between F-actin filament architecture and morphology.

(A) Swarm plots showing mean order () for each segmented filament in cells grown in static conditions (green) or under fluidic shear stress (FSS, yellow). (B) Swarm plots showing azimuth circular standard deviation (s₀) for the same groups in (A). (C) Scatter plot matrix exported from OOPS showing relationship between filament length (L) and FPM order and azimuth statistics for all filaments across both growth conditions, sorted into two groups: “Short” (blue, L < 17.5 μm) and “Long” (orange, L ≥ 17.5 μm). (D) Magnified version of the scatterplot highlighted by a black square in (C), showing the relationship between L and . A dashed black line denotes the cutoff point between “Short” and “Long” filaments, which was chosen to approximate the reported persistence length (L_p) of phalloidin-stabilized F-actin, L_p = 17.5 μm. (E–F) Same as in (A–B) but grouped based on growth condition and filament length.

doi: https://doi.org/10.1371/journal.pcbi.1011723.g005