ECOD domain classification of 48 whole proteomes from AlphaFold Structure Database using DPAM2
Fig 6
Orphan domain Duf3676 represents a fast-evolving killer toxin-like fold.
Core fold common to Duf3676 (A-C) and yeast killer toxins (D-F) are depicted in rainbow cartoon from blue (N-terminus) to red (C-terminus). Cysteine side chains (gray stick) mark the disulfides, and termini are labeled. A) Duf3676 CSS2 AF model has an N-terminal extension (slate) to the common core fold that is decorated by an edge strand from the C-terminus (magenta). B) Duf3676 representative YER076C colored as in A, with the C-terminal strand in an opposite orientation. C) Duf3676 domain with an N-terminal strand (slate) replacing the variable edge strand. D) Top identified DPAM domain (ECOD: e6greB2) from Duf26 Gnk2 homolog has a similar N-terminal edge strand (slate) as Duf3676 domain in panel C, but alternate disulfide patterns (gray stick). E) Gnk2 binds carbohydrate (black stick) near the N-terminal strand. F) Yeast killer toxin bound to carbohydrate (black stick).