Analyzing histone ChIP-seq data with a bin-based probability of being signal
Fig 5
Combining DiffBind with a PBS-based differential analysis.
(A) Volcano plot showing significantly differentially bound regions in tissue samples from two ENTEx donors (gray). Yellow highlighted points indicate regions enriched in donor 2 that also overlap with bins that have a difference in PBS > 0.9. Values have been randomly downsampled to 10000 points to facilitate visualizing trends. (B) Comparison between top GO terms for a DiffBind-based differential analysis (left) and a combined DiffBind and PBS-based analysis (right). Terms explicitly relating to immune system processes are highlighted in orange. The FDR-adjusted p values in the right panel are greater (less significant) than those in the left panel due to the smaller number of input genes.