RSim: A reference-based normalization method via rank similarity
Fig 5
Comparison of different normalization methods’ effect on the differential abundance analysis.
(a) and (b) are the FDR and sensitivity plots of the t-test after applying seven normalization methods. (c) and (d) are the FDR and sensitivity plots of the Pearson correlation test after applying seven normalization methods. The x-axis is the signal strength of differential abundant taxa. RSim can help t-test and Pearson correlation test control FDR and maintain detection power.