Automatic extraction of actin networks in plants
Fig 2
Two parallel pathways are taken from the initial image (A) after background removal and filtering. The image had vessels enhanced (B) before thresholding into a binary image (C) before being skeletonised and rotated (D). Alongside this, the initial image was separately rotated before vessel enhancement (Brt) and thresholding (Crt) followed by skeletonisation (D). The skeleton results were combined to ensure no loss of information occurred in the rotation steps. From the combined skeleton, the labelling and relabelling processes were applied (E) to identify each filament and branch for further analysis.